WebC. System Components 3 D. The pET Vectors 4 Vector Characteristics and Cloning Strategy 4 Ligation-Independent Cloning (LIC) of PCR Products 4 Fusion Tags 5 E. Antibiotic Resistance 6 F. pET Vector Characteristics 7 G. Hosts for Cloning 8 H. Hosts for Expression 8 pET System Host Strain Characteristics 9 I. Selecting Host Strains 10 WebRecombinant Human IL23R Protein with C-terminal 6×His tag Synonyms IL-23 receptor; IL-23R Delivery In Stock Uniprot ID Q5VWK5 Expression Host HEK293 Tag C-6×His tag Molecular Characterization Molecular Weight The protein has a predicted molecular mass of 37.4 kDa after removal of the signal peptide. ...
Cleavable C-terminal His-tag vectors for structure
WebThe pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information. This product is sold for internal research use only. WebArguably the simplest affinity tag is the polyhistidine (His) tag. Small and unlikely to affect function, His-tagged proteins can be purified using metal-affinity chromatography, usually using a Ni2+ column. Like other affinity … firtion-adams funeral
Recombinant Human GYPB protein(VLPs), C-His Tag
WebC-terminal tags have been shown previously to be preferable to N-terminal tags for producing some proteins , but are generally thought less attractive for structural studies … WebThe PA2694 gene coding for the Patrx2 protein with a C-terminal (His)6-tag, has been synthesized de novo and inserted into PUC57 vector using BamHI and EcoRI as restriction sites ( GenScript ). ... For each citation that was shared on social media (LinkedIn, Facebook, or Twitter) with the “@GenScript” tag, the author will be rewarded with a ... WebThe polyhistidine- or His6-tag is a protein tag originally developed for efficient protein purification in 1988 (Hochuli, Bannwarth & Döbeli et al., 1988). As a result of its comparably great cost-benefit ratio, it is known … firtion and adams